List Two Terms Used to Describe Growth in Broths

Each has specific advantages and disadvantages. If nutrient broth is transparentclear.

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It is important to protect the skin using gloves glasses and masks.

. Raised Dull Lobate or Circular See also colony morphology broth growth patterns and pigment production under additional information on the course web page. Motile bacteria those with flagella can swim. Provides big space for bacteria to grow.

Limitations and safety considerations associated with using uV light to control microorganisms. Margin The margin or edge of a colony or any growth may be an important characteristic in identifying an organism. Elevation This describes the side view of a colony.

The term used when a culture is placed at a particular temp for a period of time is X. Cells that settle to the bottom of a broth tube are referred to as _____. Limiting time and distance when working with UV light.

The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration plate on the left in the picture below. The lowest temperature at which the organism can survive and replicate is its minimum growth temperature. Common terms used to describe texture may include smooth glistening mucoid slimy dry powdery flaky etc.

Also include descriptive terms for any other relevant optical characteristics such as. On the other hand in broth culture the growthranges from sedimentation at. These are the most common.

Has more room for bacteria to grow. Some will sink to the bottom and form a sediment. Put away personal items and tie back loose hair.

List the four standard criteria omit opacitytranslucence used to describe bacterial colonies growing on agar plates. Organism TSB Escherichia coli Serratia marcescens flocculent Staphylococcus aureus Bacillus subtilis 1. A visible layer of growth at surface of broth.

The following ranges of permissive growth temperatures are approximate only. What does Pellicle growth look like. In the slant culture the bacterial growth is even to spreadingto irregular.

The highest temperature at which growth can occur is its maximum growth temperature. -A type of surface membrane that is produced by organisms floating at the top of the broth. Label the sterile broth tube name date medium and organism 2.

List the required entry procedures for the microbiology lab. Opaque cloudy translucent iridescent. The growing environment used will depend on what the researcher wants to do with or learn from the microbes.

Be sure to include the organisms names. Describe the form of growth observed in the tryptone broth tubes of your organisms 3. Different organisms will exhibit varying growth characteristics in broth.

Some organisms will diffuse uniformly throughout the broth. The growth rates are the highest at the optimum growth temperature for the organism. Growth ocuring as clumps suspended in the broth is referred to as _____.

Conclusion Result For Procedures 1 and 2 P1 P2. Use the data posted in Top Hat in the Results link to complete the table below. While holding the inoculation loop in your dominant hand flame the loop and wire from base to tip until uniformly orange-hot.

Contain chemicals that prevent growth of unwanted bacteria without inhibiting growth of desired organisms. Liquid culture where bacteria suspend in liquid substance. -Common among Mycobacterium smegmatis.

In addition to oxygen requirements there are other factors that can influence microbial growth patterns in liquid. Medium has small masses visible in suspension. Growth Patterns in Broth.

Colony Color Shape Margin Elevation Diameter mm 1 2 3 Growth in Broth Results Label the following organisms with the observed growth pattern in broth. When a bacterial culture grows across the surface of a broth the growth is referred to as a _____. Media that has a slanted surface.

Obligate Aerobe Obligate Anaerobe Facultative Anaerobe Microaerophile Media TSA in Room Air growthorne growth TSA in Gas Pak growth or no growth Thioglycollate Broth Location in the broth 4. Their movement will create a uniform cloudiness turbidity in the broth. With your free hand pick up the culture.

This is in contrast to Streptococcus species whose growth is selected against by this high salt agar plate on the right in the picture below. Shape color margin elevation. A term often used to describe cloudiness in broth media is _____.

Terms in this set 5 What are the different types of Growth Patterns in Broth. Circular plate used for culturing cells. The two main types of bacterial growth media used are liquid broth and solid Jell-o-like agar.

List 3 factors that might influence the cultural characteristics of an organism growing on agar medium. A mass of bacteria usually at the bottom of the suspension. Transparency colonies may be transparent you can see through them translucent light passes through them or opaque solid-appearing.

Some will grow in clumps producing flocculent growth and some will float on the top of the broth forming a pellicle. Describe the form of growth observed in the tryptone broth tubes of your organisms 3. Lab lecture 22.

Mannitol Salt Agar MSA This type of medium is both selective and differential. If nutrient broth is transparentclear No organisms are growing in the broth Aseptic transfer was successful Organisms are growing in the broth Aseptic transfer was unsuccessful If nutrient broth is turbid hazy or cloudy For Procedure 3 P3. UV light can not penetrate surfaces it is less effective at greater distances and can damage skin and cell.

A word meaning pigmentation or color used to describe growth on an agar slant or colony is X. Which one of the following terms might be used to describe colony shape. Other Factors That Influence Bacterial Growth in Broth.

Several examples are shown. Describe and draw the growth of your organisms on the Petri dish.

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